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Related Experiment Videos

PDGF-D, a new protease-activated growth factor.

W J LaRochelle1, M Jeffers, W F McDonald

  • 1CuraGen Corporation, 555 Long Wharf Drive, New Haven, Connecticut 06511, USA. wlaroche@curagen.com

Nature Cell Biology
|May 2, 2001
PubMed
Summary

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Researchers discovered a new Platelet-Derived Growth Factor (PDGF), named PDGF-D, which activates specific PDGF Receptors (PDGFRs). PDGF-D activates PDGFR-beta and, in combination with PDGFR-alpha, both receptors, expanding the PDGF signaling network.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Biochemistry

Background:

  • Platelet-Derived Growth Factor (PDGF) family members are crucial in development, physiology, and diseases like cancer and fibrosis.
  • Two PDGF receptors (PDGFRs) mediate PDGF signaling, with PDGF-A and PDGF-B previously identified as the sole ligands.
  • Recent discoveries, including PDGF-C, have expanded the known PDGF ligand family.

Purpose of the Study:

  • To identify and characterize a novel member of the PDGF family, designated PDGF-D.
  • To elucidate the specific interactions and activation patterns of PDGF-D with PDGFR-alpha and PDGFR-beta.

Main Methods:

  • Recombinant PDGF-D protein was purified.
  • Functional assays were performed using cells expressing PDGFRs to assess DNA synthesis and growth.

Related Experiment Videos

  • Ligand-receptor binding and activation studies were conducted on cells expressing individual or both PDGFR subtypes.

    • Main Results:

    • Purified PDGF-D stimulated DNA synthesis and growth in cells expressing PDGFRs.
    • PDGF-D selectively activated PDGFR-beta in cells expressing only PDGFR-beta.
    • In cells co-expressing PDGFR-alpha and PDGFR-beta, PDGF-D activated both receptors, suggesting potential heterodimerization.

    Conclusions:

    • A new PDGF family member, PDGF-D, has been identified and characterized.
    • PDGF-D exhibits distinct activation profiles for PDGFR-alpha and PDGFR-beta, depending on receptor co-expression.
    • The findings suggest that PDGF-D plays a role in cellular functions mediated by PDGFR signaling, potentially through receptor heterodimerization.