Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-bridge Cycle01:26

Cross-bridge Cycle

122.0K
As muscle contracts, the overlap between the thin and thick filaments increases, decreasing the length of the sarcomere—the contractile unit of the muscle—using energy in the form of ATP. At the molecular level, this is a cyclic, multistep process that involves binding and hydrolysis of ATP, and movement of actin by myosin.
122.0K
Actin and Myosin in Muscle Contraction01:16

Actin and Myosin in Muscle Contraction

20.7K
Actin and myosin are contractile proteins that form the sarcomere found in skeletal muscle tissues for regulating muscle contraction. Actin, a globular contractile protein, interacts with myosin for muscle contraction. The skeletal tissue appears striped or striated under a microscope due to the repeated arrangement of contractile proteins actin and myosin along the length of myofibrils. Dark A bands and light I bands repeat along myofibrils, and the alignment of myofibrils in the cell causes...
20.7K
Excitation-Contraction Coupling in Skeletal Muscles01:20

Excitation-Contraction Coupling in Skeletal Muscles

13.8K
Excitation-contraction coupling is a series of events that occur between generating an action potential and initiating a muscle contraction. It occurs at the triad, a structure found in skeletal muscle fibers that comprise a T-tubule and terminal cisternae of the sarcoplasmic reticulum on each side. These triads are visible in longitudinally sectioned muscle fibers. They are typically located at the A-I junction — the junction between the A and I bands of the sarcomere.
When an action...
13.8K
Overview of Myosin Structure and Function01:15

Overview of Myosin Structure and Function

6.2K
Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X)  have been well...
6.2K
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

16.6K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
16.6K
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

6.0K
In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp.

Methods in enzymology·2017
Same author

How to Measure Separations and Angles Between Intramolecular Fluorescent Markers.

Methods in enzymology·2016
Same author

Mechanical coordination in motor ensembles revealed using engineered artificial myosin filaments.

Nature nanotechnology·2015
Same author

Combining single-molecule optical trapping and small-angle x-ray scattering measurements to compute the persistence length of a protein ER/K alpha-helix.

Biophysical journal·2009
Same author

Myosin VI is a processive motor with a large step size.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle.

The Journal of biological chemistry·2001

Related Experiment Video

Updated: Jan 9, 2026

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
08:50

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

1.1K

The myosin swinging cross-bridge model.

J A Spudich1

  • 1Department of Biochemistry, Beckman Center, B400, Stanford University School of Medicine, Stanford, California 94305-5307, USA. jspudich@cmgm.stanford.edu

Nature Reviews. Molecular Cell Biology
|May 2, 2001
PubMed
Summary
This summary is machine-generated.

Myosin, an actin-based motor protein, is extensively researched using diverse scientific methods. This enzyme is now one of the most thoroughly understood in biological systems.

More Related Videos

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

2.6K
Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy
09:38

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

Published on: July 1, 2021

1.6K

Related Experiment Videos

Last Updated: Jan 9, 2026

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
08:50

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

1.1K
Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

2.6K
Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy
09:38

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

Published on: July 1, 2021

1.6K

Area of Science:

  • Biophysics
  • Biochemistry
  • Physiology
  • Genetics

Background:

  • Myosin is an essential actin-based molecular motor protein crucial for cellular functions.
  • Understanding myosin's mechanisms is vital for comprehending cellular mechanics and motility.

Purpose of the Study:

  • To highlight the multidisciplinary approaches used in myosin research.
  • To emphasize myosin's status as a well-understood enzyme in biology.

Main Methods:

  • Biophysical analyses
  • Biochemical assays
  • Physiological studies
  • Classical and molecular genetics

Main Results:

  • Myosin has been investigated through a wide array of scientific disciplines.
  • These diverse approaches have led to comprehensive knowledge of myosin's structure and function.

Conclusions:

  • The combined efforts of various scientific fields have significantly advanced our understanding of myosin.
  • Myosin serves as a model system for enzyme research due to the depth of knowledge acquired.