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Related Experiment Videos

Transfer of refrozen twice-thawed embryos do not decrease the implantation rate.

J H Check1, D Brittingham, K Swenson

  • 1The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, USA.

Clinical and Experimental Obstetrics & Gynecology
|May 3, 2001
PubMed
Summary
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Twice frozen/thawed multi-cell embryos show comparable implantation rates to once frozen embryos. This study indicates that using embryos frozen multiple times does not negatively impact pregnancy success in fertility treatments.

Area of Science:

  • Reproductive Medicine
  • Embryology
  • In Vitro Fertilization

Background:

  • Cryopreservation of human embryos is a key component of assisted reproductive technologies.
  • Assessing the viability of embryos subjected to multiple freeze-thaw cycles is crucial for optimizing treatment protocols.

Purpose of the Study:

  • To evaluate the implantation potential of human multi-cell embryos that have undergone two freeze-thaw cycles.
  • To compare the outcomes of embryo transfer cycles including twice-frozen embryos with those using only once-frozen embryos.

Main Methods:

  • A retrospective analysis of 14 frozen embryo transfer (ET) cycles involving at least one twice-frozen embryo.
  • Matching these cycles with control ET cycles using only once-frozen embryos, based on the number of embryos thawed at different developmental stages.

Related Experiment Videos

  • Comparing survival, viable pregnancy, and implantation rates between the two groups.

    • Main Results:

    • Multi-cell embryos demonstrated high survival rates after thawing, with 76.3% for once-frozen and 74.0% for twice-frozen embryos.
    • Viable pregnancy and implantation rates were 58.3% and 29.8% in cycles without twice-frozen embryos.
    • Cycles including twice-frozen embryos showed comparable rates of 50.0% for viable pregnancy and 25.5% for implantation.

    Conclusions:

    • The inclusion of twice-frozen multi-cell embryos in the embryo pool for transfer does not appear to diminish implantation rates.
    • These findings suggest that embryos can be frozen and thawed multiple times without compromising their reproductive potential.