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iPS Cell Differentiation

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Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
11:57

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Published on: July 23, 2017

Cell therapy for diabetes using piscine islet tissue.

J R Wright1, B Pohajdak

  • 1Department of Pathology, Izaak Walton Killam Health Centre, Dalhousie University Faculty of Medicine, Halifax, Nova Scotia, Canada. jwright@iwkgrace.ns.ca

Cell Transplantation
|May 3, 2001
PubMed
Summary

Tilapia islets offer a cost-effective, readily available alternative for diabetes treatment. Genetically modified tilapia produce human insulin, paving the way for potential transplantation in diabetic patients.

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Area of Science:

  • Endocrinology
  • Xenotransplantation
  • Transgenic Technology

Background:

  • Islet tissue (Brockmann bodies) in teleost fish is distinct and easily accessible, unlike mammalian pancreatic tissue.
  • Tilapia islets demonstrate successful transplantation in diabetic mice, restoring normoglycemia and normal glucose tolerance.
  • Tilapia present advantages as xenogeneic donors due to lower costs and reduced xenozoonotic risk.

Purpose of the Study:

  • To develop a novel source of insulin for potential therapeutic use in diabetic patients.
  • To create transgenic tilapia capable of producing humanized insulin.
  • To assess the feasibility of using genetically modified tilapia islets for transplantation.

Main Methods:

  • Cloning, sequencing, and site-directed mutagenesis of the tilapia insulin gene to create a humanized version.
  • Generation of founder transgenic tilapia carrying the humanized insulin gene construct.
  • Demonstration of transgene transmission and expression in offspring beta cells and serum.

Main Results:

  • Successful creation of transgenic tilapia with a "humanized" insulin gene.
  • Confirmed transmission and expression of the transgene in the first filial (F1) offspring.
  • Tilapia islets exhibit glucose responsiveness, high insulin output, cryopreservation potential, and hypoxia resistance.

Conclusions:

  • Transgenic tilapia offer a promising source for insulin production and potential islet transplantation.
  • Further genomic optimization and characterization of transgenic tilapia are necessary for clinical application.
  • Encapsulated tilapia islets could be a viable therapeutic option for type 1 diabetes.