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Related Experiment Videos

Fluorescent imaging and analysis with Typhoon 8600.

P McNamara1, W Lew, L Han

  • 1Amersham Pharmacia Biotech, Molecular Dynamics, Sunnyvale, CA 94085, USA. peter.mcnamara@am.apbiotech.com

Electrophoresis
|May 3, 2001
PubMed
Summary
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The Typhoon 8600 imager provides sensitive, quantitative laser-based detection for life science research. It enhances speed and accuracy in analyzing nucleic acids, proteins, and multicolor fluorescent signals in various assays.

Area of Science:

  • Life Science Imaging
  • Biotechnology
  • Molecular Biology

Background:

  • Laser-based imaging systems offer sensitive and quantitative detection for multiple labels in life sciences.
  • Accurate analysis of biological samples like nucleic acids and proteins is crucial for research.

Purpose of the Study:

  • To highlight the capabilities of the Typhoon 8600 variable mode imager for life science applications.
  • To demonstrate its utility in sensitive and quantitative detection of various biological samples and assays.

Main Methods:

  • Utilizing the Typhoon 8600 imager for fluorescence, storage phosphor, and chemiluminescence detection.
  • Applying the system to analyze samples in agarose and polyacrylamide gel formats.
  • Employing multicolor fluorescent signal detection for fragment analysis, electromobility shift assays, and Western blotting.

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Main Results:

  • The Typhoon 8600 system provides sensitive fluorescent detection of nucleic acids and proteins.
  • It enables accurate discrimination of multicolor fluorescent signals.
  • The imager supports various applications including fragment analysis, EMSA, and Western blotting.

Conclusions:

  • The Typhoon 8600 imager enhances the speed and accuracy of molecular analysis in life sciences.
  • Its variable mode detection capabilities make it a versatile tool for quantitative imaging.
  • The system facilitates advanced analysis of gels and blots with high sensitivity.