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Updated: Aug 8, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 12, 2010
Engineering variegated floral patterns in tobacco plants using the Arabidopsis transposable element Tag1
1Division of Biology, University of California at San Diego, La Jolla, CA 92093-0116, USA.
Abstract:
Variegated flower phenotypes were generated using the Arabidopsis transposon Tag1 and the maize R regulatory gene. Tag1 was inserted between the CaMV 35S promoter and the maize R gene and transformed into tobacco plants. In half of the transgenic plants, variegated flower patterns were observed. Each line had a different pattern, with varying intensities with three lines showing only tiny sectors indicative of late excision and one showing large sectors indicative of earlier excision.
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