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Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
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Leucodepletion and the interaction of polymorphonuclear cells with endothelium in systemic inflammatory response

K A Brown1, S M Lewis, T A Hill

  • 1Department of Immunobiology, Guy's Hospital Medical School, London, UK.

Perfusion
|May 4, 2001
PubMed

Insights

Systemic inflammatory response syndrome (SIRS) involves activated polymorphonuclear cells (PMNs) that adhere to blood vessels. Leucofiltration significantly reduces PMN adhesion, potentially mitigating SIRS complications.

Area of Science:

  • Immunology
  • Critical Care Medicine
  • Hematology

Background:

  • Polymorphonuclear cells (PMNs) abnormal interactions with blood vessels contribute to systemic inflammatory response syndrome (SIRS) and multiple organ failure.
  • Activated PMNs in SIRS patients exhibit increased CD64 and CD11b expression, decreased CD62L expression, and heightened endothelial cell adhesion.

Purpose of the Study:

  • To investigate the efficacy of leucofiltration in reducing PMN adhesion to endothelial cells in SIRS patients.
  • To assess the potential of leucofiltration as a therapeutic strategy for mitigating SIRS pathology.

Main Methods:

  • Blood from SIRS patients was processed through a laboratory-designed extracorporeal circuit with leucodepletion filters.
  • PMN activation markers (CD64, CD11b, CD62L) and adhesion to endothelial monolayers were assessed before and after leucofiltration.

Main Results:

  • Leucofiltration markedly depleted PMNs from SIRS blood.
  • Remaining PMNs showed significantly reduced binding to cultured endothelial cells compared to pre-filtration levels.

Conclusions:

  • Leucofiltration effectively reduces the number of circulating activated PMNs in SIRS.
  • This reduction in PMN adhesion may limit vascular damage and reduce the pathological manifestations of SIRS.

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