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Optimization of second-harmonic generation microscopy
R Gauderon1, P B Lukins, C J Sheppard
1Department of Physical Optics, School of Physics A28, University of Sydney, 2006, Sydney, NSW, Australia.
Micron (Oxford, England : 1993)
|May 4, 2001
Abstract:
We describe the principles and characteristics of second-harmonic generation imaging (SHGI) and explore various methods for optimization of the technique. Second-harmonic imaging is optimized for ultrashort laser pulses, high numerical aperture microscope objectives, a highly sensitive non-descanned large area detector, pseudo-phase-matching, and specimens with large second-order non linearity or which exhibit surface plasmon enhanced phenomena. We also compare and contrast the techniques of SHGI and two-photon excited fluorescence imaging.