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Related Experiment Videos

Evolution and function of the neisserial dam-replacing gene.

G Cantalupo1, C Bucci, P Salvatore

  • 1Dipartimento di Biologia e Patologia Cellulare e Moleculare L Califano, Università di Napoli Federico II, Naples, Italy.

FEBS Letters
|May 4, 2001
PubMed
Summary

Neisseria meningitidis phase variation is linked to Dam-methylase absence. A dam-replacing gene (drg) encodes a restriction enzyme (NmeBII) cleaving 5'-GmeATC-3', impacting bacterial adaptation.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Phase variation in Neisseria meningitidis is crucial for pathogenesis.
  • Homopolymeric tracts are key sites for phase variation.
  • Dam-methylase absence influences phase variation mechanisms.

Purpose of the Study:

  • To investigate the role of the dam-replacing gene (drg) in Neisseria meningitidis.
  • To characterize the enzymatic activity of the drg gene product.
  • To explore the presence and function of drg in Neisseria lactamica.

Main Methods:

  • Gene replacement experiments in Dam-defective strains.
  • Enzyme characterization of the drg gene product.
  • Sequence analysis of Neisseria lactamica strains.

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Main Results:

  • The drg gene encodes a restriction endonuclease, NmeBII, which cleaves 5 '-GmeATC-3 '.
  • drg is present in Neisseria lactamica but often inactive due to promoter absence.
  • Inactive drg in N. lactamica is linked to GATmeC, suggesting an alternative RM system.

Conclusions:

  • NmeBII, encoded by drg, is a novel restriction enzyme involved in Neisseria meningitidis phase variation.
  • Alternative restriction-modification systems may exist in Neisseria species.
  • Understanding these systems provides insights into bacterial genome dynamics and adaptation.