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Updated: Jun 18, 2026
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Published on: December 28, 2010
A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease
T B Kristiansen1, T B Knudsen, S Ohlendorff
1Clinical Research Unit 136, Copenhagen University Hospitals Hvidovre, Kettegaard Alle 30, 2650 Hvidovre, Copenhagen, Denmark.
Abstract:
The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.
