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Updated: Jul 27, 2026

Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells
Published on: March 8, 2015
Rat retinal progenitor cells and a retinal pigment epithelial factor
H J Sheedlo1, A M Brun-Zinkernagel, L X Oakford
1Department of Pathology and Anatomy, Division of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. hsheedlo@hsc.unt.edu
Abstract:
Retinal progenitor cells were isolated from explants of neonatal rat retinas and characterized by transmission electron microscopy and reverse transcriptase-polymerase chain reaction and by their response to an isolated retinal pigment epithelial cell cell factor. The isolated progenitor cells demonstrated nuclei with abundant euchromatin typical of progenitor cells and showed the presence of nestin and opsin message. A protein ( approximately 67 kDa) isolated from conditioned media of cultured rat RPE cells promoted the survival of isolated retinal progenitor cells.
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