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Related Experiment Videos

Three-dimensional structure of the anaphase-promoting complex.

C Gieffers1, P Dube, J R Harris

  • 1Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.

Molecular Cell
|May 5, 2001
PubMed
Summary

The anaphase-promoting complex (APC), a key cell cycle regulator, has a complex 3D structure revealed by cryo-EM. This structure suggests a central cavity may function as a reaction chamber for ubiquitination.

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Area of Science:

  • Cell Biology
  • Structural Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex (APC) is a crucial ubiquitin-protein ligase regulating cell cycle progression through mitosis and G1.
  • APC is composed of at least 11 subunits, highlighting its complex nature.

Purpose of the Study:

  • To determine the three-dimensional structure of the human anaphase-promoting complex (APC).
  • To elucidate the structural basis for APC function in cell cycle regulation.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) was employed to visualize the APC.
  • Angular reconstitution techniques were used to generate a 3D model.

Main Results:

  • A high-resolution (24 Å) three-dimensional model of the human APC was obtained.

Related Experiment Videos

  • The APC exhibits a complex asymmetric structure (140 Å x 140 Å x 135 Å) with an outer wall and a large inner cavity.
  • The structure suggests the inner cavity may serve as a reaction chamber for ubiquitination.

    • Conclusions:

    • The determined 3D structure provides new insights into the architecture of the APC.
    • The proposed 'cage hypothesis' suggests the inner cavity facilitates ubiquitination reactions, potentially explaining the APC's subunit complexity.