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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
Published on: April 7, 2012
PROBING PLANT METABOLISM WITH NMR
R George Ratcliffe1, Yair Shachar-Hill
1Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, United Kingdom; e-mail: george.ratcliffe@plants.ox.ac.uk, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003;
Annual Review of Plant Physiology and Plant Molecular Biology
|May 5, 2001
Summary
Nuclear magnetic resonance (NMR) spectroscopy offers powerful analytical methods for understanding plant metabolism. This technique aids in identifying, quantifying, and localizing metabolites, crucial for metabolic engineering and functional genomics.
Area of Science:
- Plant science
- Biochemistry
- Analytical chemistry
Background:
- Functional genomics and metabolic engineering necessitate advanced methods for studying plant metabolism.
- Nuclear magnetic resonance (NMR) spectroscopy provides unique insights into metabolic integration and regulation.
Purpose of the Study:
- To review the applications of NMR spectroscopy in plant metabolism research.
- To highlight the significance of NMR in understanding metabolic pathways and phenotypes.
Main Methods:
- In vivo and in vitro NMR measurements.
- Metabolite identification, quantification, and localization using NMR.
- NMR applications in metabolic flux analysis, metabolite profiling, and imaging.
Main Results:
- NMR enables detailed exploration of intermediary carbon and nitrogen metabolism.
- The technique is valuable for defining the intracellular environment and pathway operation.
- NMR facilitates the characterization of metabolic phenotypes.
Conclusions:
- Metabolic NMR spectroscopy plays a vital role in advancing quantitative understanding of plant metabolism.
- NMR is essential for the characterization of metabolic phenotypes in plants.
- The continued application of NMR is crucial for progress in plant metabolic research.
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