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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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Determination of substrate and product concentrations in lactic acid bacterial fermentations by proton NMR using the

V Silvestre1, S Goupry, M Trierweiler

  • 1LAIEM-UMR CNRS 6006, Faculté des Sciences, Université de Nantes, 2 rue de la Houssiniere, BP 92208, 44322 Nantes, France.

Analytical Chemistry
|May 8, 2001
PubMed

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Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
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