Thiophilic interaction chromatography of prostate-specific antigen
K C Chadha1, E Kawinski, E Sulkowski
1Roswell Park Cancer Institute, Department of Molecular and Cellular Biology, Buffalo, NY 14263, USA. kailash.chadha@roswellpark.org
Abstract:
Prostate-specific antigen (PSA) protein and complexes of PSA with alpha1-antichymotrypsin (PSA-ACT) or alpha2-macroglobulin (PSA-A2M) prepared in vitro, have strong affinity for different thiophilic gels (T-gel). Free PSA, and these PSA complexes can be isolated due to their affinity for T-gels. The average recovery of PSA from several of the T-gels, based upon ELISA measurements, was 84 to 94%. The data suggest that T-gel affinity can be explored for the purification of free and complexed PSA from various biologic fluids.
