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Related Experiment Videos

Hyaluronidase can modulate expression of CD44.

R Stern1, S Shuster, T S Wiley

  • 1Department of Pathology, School of Medicine, University of California, San Francisco, California 94143-0506, USA. rstern@itsa.ucsf.edi

Experimental Cell Research
|May 8, 2001
PubMed
Summary
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Hyaluronan influences CD44 splice variant selection in cancer cells. Variant CD44 isoforms are sensitive to hyaluronidase, while standard CD44 is resistant, suggesting differential stability and ligand dependence.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • CD44 is a transmembrane glycoprotein family with diverse isoforms generated by alternative splicing.
  • CD44 variants and hyaluronan presence are implicated in cancer cell motility, invasion, and metastasis.
  • Mechanisms governing CD44 splice variant selection remain largely unknown.

Purpose of the Study:

  • To investigate the impact of hyaluronidase digestion on CD44 expression in human cancer cell lines.
  • To explore the relationship between hyaluronan and CD44 splice variant stability.
  • To identify novel CD44 variant isoforms.

Main Methods:

  • Treatment of human cancer cell lines with hyaluronidase.
  • Analysis of CD44 isoform expression following digestion.

Related Experiment Videos

  • Observation of differential sensitivity of CD44 variants to hyaluronidase.

    • Main Results:

    • CD44 isoforms with alternatively spliced exons showed sensitivity to hyaluronidase digestion across all cell lines.
    • Standard CD44 (CD44s) expression was resistant to hyaluronidase digestion in two of three cell lines.
    • New CD44 variant isoforms, previously unobserved, were identified.

    Conclusions:

    • A model is proposed where CD44 splice variants are unstable and require continuous ligand (hyaluronan) for expression.
    • CD44s is relatively more stable and does not require continuous hyaluronan presence.
    • Hyaluronan plays a critical role in regulating the stability and expression of CD44 isoforms in cancer cells.