Optical peroxide biosensor using the electrically controlled-release technique

Area of Science:

• Biomedical Engineering
• Chemical Sensors
• Analytical Chemistry

Background:

• Peroxide concentration measurement is crucial in various applications.
• Existing biosensors may lack precise control over reagent release.
• Development of novel biosensing platforms is needed.

Purpose of the Study:

• To develop an optical biosensor for peroxide concentration measurement.
• To implement an electrically controlled-release system for precise reagent delivery.
• To characterize the performance and analytical capabilities of the developed biosensor.

Main Methods:

• Fabrication of an optical biosensor incorporating an electrically controlled-release system.
• Utilizing a polymer complex responsive to pH changes induced by electric current for reagent release.
• Enzyme-based detection of peroxide using 4-hydroxyphenylacetic acid (HPA) and fluorescence detection of the product DBDA.

Main Results:

• The electrically controlled-release system successfully released HPA proportionally to the applied electric current.
• A model was developed to predict the degradation velocity of the polymer complex and HPA release rate.
• The biosensor demonstrated a linear analytical range of 0.025–1.0 mM for peroxide with a 20-minute response time.

Conclusions:

• The developed optical biosensor with an electrically controlled-release system offers a reliable method for peroxide detection.
• The precise control over reagent release enhances the accuracy and efficiency of peroxide measurements.
• The biosensor exhibits good repeatability and reproducibility, indicating its potential for practical applications.