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Fast sequential injection determination of benzo[A]pyrene using variable angle fluorescence with on-line solid-phase

J A Erustes1, A Andrade-Eiroa, A Cladera

  • 1Department of Chemistry, University of Balearic Islands, E-07071 Palma de Mallorca, Spain.

The Analyst
|May 9, 2001
PubMed
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A new method efficiently analyzes benzo[a]pyrene (BaP), a potent carcinogen, in drinking water. This technique achieves low detection limits, meeting strict regulatory standards for public consumption.

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry

Background:

  • Benzo[a]pyrene (BaP) is a potent carcinogenic polycyclic aromatic hydrocarbon (PAH).
  • Accurate analysis of BaP in drinking water is crucial for public health and regulatory compliance.

Purpose of the Study:

  • To develop and validate a sensitive methodology for quantifying benzo[a]pyrene (BaP) in drinking water.
  • To ensure the method meets the stringent requirements of the 98/83/EC Directive for drinking water quality.

Main Methods:

  • Sequential injection analysis utilizing a C18 microcolumn for BaP extraction and preconcentration.
  • Elution with a small volume of 1,4-dioxane followed by variable angle fluorescence detection.
  • Data processing using multiple linear regression for spectral analysis.

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Main Results:

  • Achieved a detection limit of 2.5 ng l-1 for BaP, well below the regulatory limit.
  • Established a linear range for BaP quantification from 7.5 to 280 ng l-1.
  • Demonstrated the reusability of the microcolumn and the efficiency of small sample and solvent volumes.

Conclusions:

  • The developed methodology provides a reliable and efficient means for determining BaP in drinking water.
  • The method's sensitivity and efficiency comply with European Union drinking water directives.
  • This approach offers advantages in terms of cost-effectiveness and reduced solvent consumption.