Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ADAM8: a novel osteoclast stimulating factor.

S J Choi1, J H Han, G D Roodman

  • 1Department of Medicine/Hematology, University of Texas Health Science Center, San Antonio, USA.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|May 9, 2001
PubMed
Summary

ADAM8 significantly impacts osteoclast (OCL) formation, a key process in bone remodeling. This gene is crucial during later OCL differentiation stages, influencing bone resorption.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Deepening the Action on Salt Reduction in China-suggestions on strategy and implementation plan].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2023
Same author

Analysis of synthetic electron cyclotron emission from the high field side of HL-2M tokamak plasmas.

The Review of scientific instruments·2022
Same author

The global burden of sudden infant death syndrome from 1990 to 2019: a systematic analysis from the Global Burden of Disease study 2019.

QJM : monthly journal of the Association of Physicians·2022
Same author

Harlequin syndrome with petechiae caused by a pituitary adenoma.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

3D wide-field multispectral photoacoustic imaging of human melanomas in vivo: a pilot study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

Fate of the micropenis and constitutional small penis: do they grow to normalcy in puberty?

Journal of pediatric urology·2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteoclasts (OCLs) are critical for bone resorption and remodeling.
  • Identifying genes involved in OCL differentiation is essential for understanding bone homeostasis and diseases.

Purpose of the Study:

  • To identify genes highly expressed in OCLs compared to their precursors.
  • To investigate the role of ADAM8 in the OCL differentiation process.

Main Methods:

  • Polymerase chain reaction (PCR)-selective complementary DNA (cDNA) subtraction hybridization.
  • Antisense (AS) S-oligonucleotides to inhibit ADAM8 expression.
  • Conditioned media from transfected cells to assess ADAM8 activity.
  • Western blot analysis.

Related Experiment Videos

Main Results:

  • ADAM8 was identified as a gene highly expressed in OCLs.
  • Inhibition of ADAM8 significantly reduced OCL formation.
  • Soluble ADAM8 stimulated OCL formation and bone pit formation.
  • ADAM8 activity was critical during the later stages of OCL differentiation (days 4-7).
  • The cysteine-rich/disintegrin domain of ADAM8 mediated its stimulatory activity.

Conclusions:

  • ADAM8 plays a significant role in OCL formation and differentiation.
  • ADAM8 acts primarily in the later stages of OCL development.
  • The findings suggest ADAM8 as a potential therapeutic target for bone diseases.