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Image analysis for quantitation of solid tumor drug sensitivity
1Department of Surgical Oncology, Roswell Park Cancer Institute, State University of New York at Buffalo, 14263, USA. jgibbs@sc3101.med.buffalo.edu
International Journal of Surgical Investigation
|May 9, 2001
Summary
This study introduces an image analysis system to rapidly assess drug effects on tumor growth. The system quantitatively measures 5-Fluorouracil (5-FU) chemosensitivity in colon adenocarcinoma, showing promising results for in vitro drug testing.
Area of Science:
- Oncology
- Biotechnology
- Medical Imaging
Background:
- Established methods for assessing tissue chemosensitivity exist.
- The study aimed to improve speed and quantification in drug effect assessment on tissue proliferation.
Purpose of the Study:
- To develop a rapid, quantitative image analysis system for evaluating drug effects on tumor proliferative capacity.
- To assess the chemosensitivity of colon adenocarcinoma to 5-Fluorouracil (5-FU) in vitro.
Main Methods:
- Ward rat colon adenocarcinoma xenografts were cultured on collagen-cellulose matrices at an air-fluid interface.
- Image analysis quantified tumor area and fluorescent brightness using a low-light detecting camera and image processing unit.
- Tumors were treated with varying concentrations of 5-Fluorouracil (5-FU), with measurements taken pre- and post-treatment.
Main Results:
- Nontreated tumors showed increased area and fluorescent brightness over time.
- A dose-dependent decrease in tumor area was observed with increasing 5-FU concentrations.
- A 39% decrease in tumor area was noted at the highest clinically achievable 5-FU concentration (500 microM).
Conclusions:
- The developed image analysis system effectively demonstrated fluoropyrimidine activity.
- In vitro 5-FU sensitivity in collagen gel cultures correlated with in vivo tumor responsiveness.