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Isolation and characterization of a human thiamine pyrophosphokinase cDNA
K Nosaka1, M Onozuka, N Kakazu
1Department of Chemistry, Kyoto Prefectural University of Medicine, Kita-ku, Kyoto 603-8334, Japan. knosaka@basic.kpu-m.ac.jp
Biochimica Et Biophysica Acta
|May 9, 2001
Abstract:
A human thiamine pyrophosphokinase cDNA clone (hTPK1) was isolated and sequenced. When the intact hTPK1 open reading frame was expressed as a histidine-tag fusion protein in Escherichia coli, marked enzyme activity was detected in the bacterial cells. The hTPK1 mRNA was widely expressed in various human tissues at a very low level, and the mRNA content in cultured fibroblasts was unaffected by the thiamine concentration of the medium. The chromosome localization of the hTPK1 gene was assigned to 7q34.