Related Experiment Video
Updated: Oct 7, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Characterization and transcriptional analysis of Pseudomonas fluorescens denitrifying clusters containing the nar,
L Philippot1, P Mirleau, S Mazurier
1Institut National de la Recherche Agronomique-Laboratoire de Microbiologie des Sols et de Recherche sur la Flore Pathogène, 17, rue Sully, B.V. 1540, 21034 Cedex, Dijon, France. philippo@dijon.inra.fr
Abstract:
In this study, we report the cloning and characterization of denitrifying gene clusters of Pseudomonas fluorescens C7R12 containing the narXLDKGHJI, nirPOQSM, norCB and nosRZDFYL genes. While consensus sequences for Fnr-like protein binding sites were identified in the promoter regions of the nar, nir, nor and nos genes, consensus sequences corresponding to the NarL binding sites were identified only upstream the nar genes. Monitoring by mRNA analysis the expression of the narG, nirS, norB and nosZ structural genes suggests a sequential induction of the denitrification system in P. fluorescens.
More Related Videos
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
10:18A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
Published on: April 25, 2015
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial Transcription
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are: