Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear pores and nuclear assembly.

S K Vasu1, D J Forbes

  • 1Section of Cell and Developmental Biology, Division of Biology, University of California San Diego, La Jolla, CA 92093-0347, USA.

Current Opinion in Cell Biology
|May 10, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Welcome to the nucleus: CAN I take your coat?

Nature cell biology·2002
Same author

Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.

The Journal of cell biology·2001
Same author

Purification of the vertebrate nuclear pore complex by biochemical criteria.

Traffic (Copenhagen, Denmark)·2001
Same author

Identification of a new vertebrate nucleoporin, Nup188, with the use of a novel organelle trap assay.

Molecular biology of the cell·2000
Same author

The nucleoporin nup153 plays a critical role in multiple types of nuclear export.

Molecular biology of the cell·1999
Same author

Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors.

Current biology : CB·1999

Nuclear pores, crucial for cell communication, have been purified and their transport routes elucidated. New roles for the GTPase Ran in nuclear processes were also revealed.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • Nuclear pores are essential macromolecular structures facilitating communication between the nucleus and cytoplasm.
  • Previous estimates placed the mass of nuclear pores at 60 million Daltons (yeast) and 120 million Daltons (vertebrates).

Purpose of the Study:

  • To summarize recent advancements in nuclear pore research.
  • To highlight the purification and characterization of nuclear pore complexes.
  • To explore the functional roles of nuclear pores and associated proteins.

Main Methods:

  • Quantitative scanning transmission electron microscopy was used for mass estimation.
  • Purification techniques were applied to yeast and vertebrate nuclear pores.
  • Studies involved describing transport receptor routes and observing intranuclear organization.

Related Experiment Videos

Main Results:

  • Successful purification of both yeast and vertebrate nuclear pores.
  • Initial characterization of pathways for specific transport receptors through nuclear pores.
  • Insights into intranuclear organization influenced by nuclear pores and the nuclear envelope.
  • Discovery of significant roles for the small GTPase Ran in nuclear import, spindle assembly, and nuclear membrane fusion.

Conclusions:

  • Recent advances have significantly improved our understanding of nuclear pore structure and function.
  • The small GTPase Ran plays a multifaceted role in critical cellular processes beyond nuclear import.