Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Measuring the diaspora for virus-specific CD8+ T cells.

D R Marshall1, S J Turner, G T Belz

  • 1Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 10, 2001
PubMed
Summary

Following influenza A virus infection, virus-specific CD8(+) T cells are widely dispersed throughout the body, with varying activation markers, indicating heterogeneous memory cell populations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Global prevalence of FGFR2b protein overexpression in advanced gastric cancer and gastroesophageal junction cancers: pooled analysis of two bemarituzumab phase III studies.

ESMO open·2026
Same author

A phase 2 multicenter, prospective, randomized, double-blind study to assess the clinical and antiviral efficacy and safety of nitazoxanide for the treatment of norovirus in hematopoietic stem cell and solid organ transplant recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Molecular and physiological acclimation to low light and iron scarcity in a globally abundant oceanic pelagophyte.

Nature communications·2026
Same author

The ASSIST CLAD study: A phase 2 randomized controlled trial of mesenchymal stromal cells for new-onset chronic lung allograft dysfunction.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026
Same author

Effect of Orem-based Nursing Intervention with Mobile Application on the Symptoms, Quality of Life, and Self-Care Abilities of Gastrointestinal Cancer Patients; A Quasi-Experimental Study.

Nigerian journal of clinical practice·2026
Same author

Beyond the surface: a case report of penile tuberculosis as the first sign of miliary disease in a solid organ transplant recipient.

ASM case reports·2026

Area of Science:

  • Immunology
  • Virology
  • T cell biology

Background:

  • Understanding the distribution and phenotype of memory CD8(+) T cells after viral infection is crucial for vaccine development and immunotherapy.
  • Previous studies have focused on T cell responses in specific tissues, but a comprehensive analysis of their systemic distribution post-infection is lacking.

Purpose of the Study:

  • To analyze the systemic distribution and phenotype of virus-specific CD8(+) T cells following a secondary challenge with influenza A virus.
  • To investigate the relationship between clonal expansion, memory pool size, and tissue-specific T cell accumulation.
  • To characterize the phenotypic heterogeneity of resting memory CD8(+) T cells in different organs.

Main Methods:

  • Mice were secondarily challenged with influenza A virus.

Related Experiment Videos

  • Numbers of D(b)NP(366)- and D(b)PA(224)-specific CD8(+) T cells were quantified using tetramer staining.
  • T cell populations were analyzed sequentially in various organs including the lung, lymph nodes, spleen, blood, bone marrow, and liver over a 4-month period.

    • Main Results:

    • Virus-specific CD8(+) T cell counts decreased only twofold systemically over 4 weeks, eventually plateauing after 2 months, despite rapid declines in the lung and mediastinal lymph nodes.
    • The spleen and bone marrow harbored the largest numbers of virus-specific CD8(+) T cells, which remained significantly elevated for at least 4 months.
    • Phenotypic analysis revealed heterogeneity in CD69 expression, with lowest levels in blood and spleen, intermediate levels in bone marrow and liver, and high levels in regional lymph nodes and nasal-associated lymphoid tissue.

    Conclusions:

    • Resting memory CD8(+) T cell populations are widely dispersed throughout the body, not confined to specific tissues.
    • The distribution and phenotype of memory CD8(+) T cells are influenced by homeostatic and local environmental factors, irrespective of epitope specificity.
    • This systemic heterogeneity has implications for understanding immune surveillance and the efficacy of T cell-mediated immunity.