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Immunodetection of living trophoblast
1Department of Obstetrics and Gynecology, Poriya Government Hospital, Tiberias, Israel.
Background:
Human chorionic gonadotropin, the pregnancy hormone, is synthesized by trophoblast cells which make up the placenta.
Objective:
To determine whether antibody to hCG can be used to specifically detect living trophoblast in vitro by binding to the external membrane.
Methods:
Trophoblast was isolated from fresh placentas of women undergoing termination of pregnancy in the first trimester and incubated with monoclonal antibody to hCG. Anti-mouse immunoglobulin G with a fluorescent marker was then added.
Results:
Syncytiotrophoblast stained positive on the external surface of the cell, while controls of leukocytes, endometrial cells and hepatocytes were negative.
Conclusion:
The hCG monoclonal antibody may be used to specifically detect hCG on the surface of living trophoblast in vitro.