Related Experiment Video
Updated: Oct 7, 2026
A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
[Extrarenal effects of furosemide on normal and ischemic cerebral tissue in the rat]
C Oria De Suárez1, A Eblen Zajjur
1Dpto.de Ciencias Fisiológicas y Biofísica; Universidad de Carabobo. Facultad de Ciencias de la Salud., Valencia, Venezuela. coria@uc.edu.ve
Introduction:
Recent studies suggest that furosemide (FUR), a chloride cotransporting antagonist, acts directly on neurons of central nervous system modifying the ionic flow.
Objective:
The aim of the present study was to determine the extrarenal antiedema effect of FUR.
Material And Methods:
Male Sprague-Dawley rats were anesthetized with tiobarbital (60 mg/kg-1 ip), bilaterally nephrectomized and perfunded iv with solution of Ringer (RIN) (n= 5) or FUR (40 mg/kg-1) (n= 6), in equivalent volumes. The diuretic effect of FUR was excluded by bilateral nephrectomy. Two tungsten microelectrodes implanted on the cerebral cortex, registered the tissue impedance by the voltage fall of a square wave electric pulse (100 mA, 10 ms) tested every 120 seconds, before and after infusion of FUR or RIN and during global cerebral ischemia by D-tubocurarine induced respiratory arrest.
Results:
Preischemic FUR infusion induced a significant increase of voltage (t= 15.68; p< 0.0001). During global cerebral ischemia, the voltage fall was significantly lower in FUR than for RIN infusion (Wilcoxon Z= 3.9; p< 0.00001).
Conclusion:
These results suggest that FUR reduced significantly the cerebral impedance i.e. antiedema effect, by an extrarenal action, under both basal and ischemia conditions.