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Molecular identification of TEM-1 beta-lactamase in a Pasteurella multocida isolate of human origin
T Naas1, F Benaoudia, L Lebrun
1Service de Bactériologie-Virologie, H pital de Bicêtre, Le Kremlin-Bicêtre, France. thierry.naas@kb.u-psud.fr
Abstract:
Two clinical strains of Pasteurella multocida were isolated from an HIV-infected patient who developed arthritis. Strain FB-1, which was isolated from a dog-bite wound, was resistant to narrow-spectrum penicillins. The second strain, FB-2, which was present in blood cultures as well as the dog-bite wound, was susceptible to all beta-lactam agents. Arbitrarily primedpolymerase chain reaction and pulsed-field gel electrophoresis revealed that these two isolates were genetically indistinguishable. 16S rDNA gene sequencing facilitated identification at the subspecies level. Amoxicillin resistance determinant was located on a highly unstable 4.3-kb plasmid, pFAB-1. Isoelectrofocusing and polymerase chain reaction amplification followed by sequencing revealed the presence of a pI 5.4 TEM-1 beta-lactamase. This description is the first of a TEM-1 Beta-lactamase' in a Pasteurella multocida strain of human origin.
Insights
Two Pasteurella multocida strains from an HIV patient showed genetic similarity. One strain harbored a TEM-1 beta-lactamase, a first for human-origin Pasteurella multocida, linked to amoxicillin resistance.
Area of Science:
- Microbiology
- Infectious Diseases
- Genetics
Background:
- Pasteurella multocida is an opportunistic pathogen.
- Infections can occur following animal bites or scratches.
- HIV-infected individuals may be more susceptible to infections.
Observation:
- Two genetically indistinguishable Pasteurella multocida strains were isolated from an HIV-infected patient with arthritis.
- Strain FB-1, from a dog-bite wound, showed resistance to narrow-spectrum penicillins.
- Strain FB-2, found in blood and wound, was susceptible to all beta-lactam agents.
Findings:
- Arbitrarily primed PCR and PFGE confirmed isolates were genetically identical.
- 16S rDNA sequencing confirmed subspecies identification.
- A TEM-1 beta-lactamase, conferring amoxicillin resistance, was identified on plasmid pFAB-1 in strain FB-1. This is the first report of TEM-1 beta-lactamase in human-origin Pasteurella multocida.
Implications:
- This finding highlights the potential for antibiotic resistance in Pasteurella multocida of human origin.
- Understanding resistance mechanisms is crucial for effective treatment of Pasteurella multocida infections, especially in immunocompromised patients.
- The presence of transferable resistance genes on plasmids warrants further investigation.