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Tailor-made dyes for fluorescence correlation spectroscopy (FCS)
Biological Chemistry
|May 12, 2001
Abstract:
Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.