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Method development for the high-performance liquid chromatographic enantioseparation of 2-cyanocycloalkanols
1Department of Inorganic and Analytical Chemistry, University of Szeged, Hungary.
Journal of Chromatographic Science
|May 12, 2001
Abstract:
An indirect high-performance liquid chromatographic method is developed for the enantioseparation of cis- and trans-2-cyanocyclopentanol and -cyclohexanol. The racemic cis-(1S,2S and 1R,2R)- or trans-(1S,2R and 1R,2S)-2-cyanocycloalkanols are converted to their diastereomers formed with (S)-(+)- or (R)-(-)-alpha-methoxy-alpha-(trifluoromethyl)phenylacetyl chloride. The diastereomers are separated on a reversed-phase column, and the conditions of derivatization and HPLC analysis are optimized.