Related Experiment Video
Updated: Aug 16, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
3'-nitrophenylpropyloxycarbonyl (NPPOC) protecting groups for high-fidelity automated 5' --> 3' photochemical DNA
M C Pirrung1, L Wang, M P Montague-Smith
1Department of Chemistry, Levine Science Research Center, Duke University, Box 90317, Durham, North Carolina 27708, USA. pirrung@chem.duke.edu
Abstract:
[structure: see text]. The most powerful DNA microarrays would be prepared by photolithography with free 3'-ends that could be processed enzymatically. A photoremovable group that could be removed in quantitative yield would ensure high purity of the synthesized probes. We have developed new pyrimidine building blocks for 5' --> 3' DNA synthesis with high cycle yields using the NPPOC (3'-nitrophenylpropyloxycarbonyl) protecting group. These phosphoramidites were proved in automated photochemical DNA synthesis on a modified synthesizer.
More Related Videos
Related Concept Videos
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Biosynthesis of Nucleic Acids

