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Intrastrand self-complementary sequences in Bacillus subtilis DNA.

D A Galloway, R Rudner

    Journal of General Microbiology
    |April 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers isolated biologically active intrastrand self-complementary DNA sequences from Bacillus subtilis. These DNA structures showed transforming activity and hybridized with RNA, indicating potential biological functions.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Intrastrand self-complementary DNA sequences can form secondary structures within a single DNA strand.
    • Bacillus subtilis is a well-studied bacterium with a sequenced genome, making it a model organism for genetic studies.

    Purpose of the Study:

    • To isolate and characterize intrastrand self-complementary DNA sequences from Bacillus subtilis.
    • To investigate the biological activity and functional relevance of these DNA structures.

    Main Methods:

    • Hydroxyapatite (HA) chromatography was used to isolate DNA fragments after thermal renaturation.
    • Chromatography on methylated albumin kieselguhr (MAK) was employed to separate DNA strands.
    • S1 nuclease was used to remove single-stranded DNA regions.

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  • Biological activity was assessed through transformation assays and hybridization studies with RNA.

    • Main Results:

    • Intrastrand self-complementary DNA structures (HA HII) were successfully isolated from Bacillus subtilis.
    • These structures exhibited biological activity, including transforming activity for various markers.
    • The DNA demonstrated hybridization capabilities with both pulse-labeled and ribosomal RNA.
    • Treatment with S1 nuclease did not significantly affect the biological activity of the self-annealed molecules.

    Conclusions:

    • The isolated intrastrand self-complementary DNA sequences from Bacillus subtilis possess biological activity.
    • These sequences are involved in DNA transformation and interact with RNA, suggesting roles in gene expression or regulation.
    • The low efficiency of transformation and hybridization indicates that only a subset of these sequences is functionally active in the studied contexts.