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Established cell line sensitive to influenza C virus
The Journal of General Virology
|April 1, 1979
Summary
Influenza C virus efficiently grows in LLCMK2 monkey kidney cells. Adding trypsin to the cell culture medium significantly enhances influenza C virus yields and plaque formation for improved infectivity assays.
Area of Science:
- Virology
- Cell Biology
Background:
- Influenza C virus is a significant human respiratory pathogen.
- Efficient cell culture methods are crucial for influenza virus research and diagnostics.
Purpose of the Study:
- To investigate the productive growth of influenza C virus in a specific cell line.
- To evaluate the effect of trypsin on virus yield and infectivity.
Main Methods:
- Utilized LLCMK2 monkey kidney cells for influenza C virus culture.
- Assessed virus growth with and without trypsin supplementation.
- Employed plaque assays in LLCMK2 cells for infectivity determination.
Main Results:
- Influenza C virus strains grew productively in LLCMK2 cells without adaptation.
- Trypsin addition to the medium increased virus yields compared to other cell cultures.
- Plaque assays in LLCMK2 cells demonstrated higher infectivity than traditional methods.
Conclusions:
- LLCMK2 cells provide an effective system for influenza C virus propagation.
- Trypsin is essential for optimizing influenza C virus yields and plaque assays.
- Plaque assay in LLCMK2 cells offers a sensitive method for influenza C virus infectivity assessment.