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[Relationship between the dynamic expression of scavenger receptor on Kupffer cells and endotoxin-induced hepatic
Objective:
To observe the change of scavenger receptor (SR) expression on Kupffer cells and study its role in pathogenesis of liver injury in mice.
Methods:
1mg/kg or 10 mg/kg of E.Coli 026: B6 LPS was bolus injected via i.a.to reproduce an animal model for this experiment. The change of SR on Kupffer cells was determined with immunohistochemistry. TNF-alpha and IL-6 level in hepatic tissue were assayed using ELISA.
Results:
The expression of SR on Kupffer cells was aggressively downregulated in a LPS does dependent fashion. TNF-alpha, IL-6 level in hepatic tissue and ALT, TBIL in plasma were significantly negatively correlated with the expression of SR.
Conclusions:
With the down-regulation of SR expression on Kupffer cells, the clearance of endotoxin by Kupffer cells is decreased and the activation of Kupffer cells by endotoxin is accordingly enhanced. SR expression is closely related to hepatic injury.