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Related Experiment Video

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Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
08:06

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Synapsin I identified as a novel brain-specific autoantigen.

V M Gitlits1, J W Sentry, L S Matthew

  • 1Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, Australia.

Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research
|May 16, 2001
PubMed
Summary

Researchers identified a novel brain autoantigen as synapsin I. Autoantibodies targeting synapsin I were found in patients with lupus and other autoimmune conditions, not just discoid lupus erythematosus.

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Area of Science:

  • Neuroimmunology
  • Autoimmunity research

Background:

  • A novel 74-kd brain-specific autoantigen was identified.
  • This autoantigen reacted with serum from a patient with discoid lupus erythematosus and chronic lymphocytic leukemia.

Purpose of the Study:

  • To identify and characterize a novel brain-specific autoantigen.
  • To determine the molecular properties and identity of the autoantigen.
  • To investigate the reactivity of autoantibodies against this antigen.

Main Methods:

  • Determined molecular weight, tissue, and subcellular distribution of the autoantigen.
  • Purified the autoantigen using ion-exchange chromatography and trypsin digestion.
  • Obtained limited amino acid sequence for comparison.

Main Results:

  • The 74-kd autoantigen was identified as synapsin I based on molecular weight, brain-specific distribution, subcellular localization, and microtubule association.
  • Amino acid sequencing confirmed identity with synapsin I.
  • Autoimmune serum immunoblotted fusion proteins of rat synapsin Ia, with autoantibodies of IgG and IgM classes detected.

Conclusions:

  • This is the first report of autoantibodies reactive to synapsin Ia.
  • Reactivity to synapsin Ia is not exclusive to discoid lupus erythematosus, as it was found in systemic lupus erythematosus and rheumatoid factor-positive sera.
  • The association between autoantibodies to synapsin I and neuropsychiatric manifestations remains undetermined.