Phospholipase C activity from Catharanthus roseus transformed roots: aluminum effect
M L Piña-Chable1, S M Herñandez-Sotomayor
1Unidad de Biología, Experimental, Centro de Investigación Científica de Yucatán Apdo, Cordemex, Mexico.
Abstract:
The effect of aluminum on the activity of PLC was examined in transformed roots from Catharanthus roseus (L) G. Don. When added in vitro to the reaction mixture, Al inhibited the enzymatic activity in a concentration and time-dependent fashion. This effect is very similar for both activities (soluble and membrane-associated). When roots were treated in vivo with Al 0.1 mM for short periods (0-4 h), PLC activity was also inhibited. Aluminum (1 mM) diminished root growth in approximately 50% when added on the first day of the culture cycle conditions in which PLC activity is also affected. Other enzymatic activities (NAD+-GDH, NADH-GDH, NADH-GOGAT and HMGR) were not affected when roots were treated with Al (0.1 mM) for short periods of time (1 h). Results obtained in this work suggest that the Al can affect PLC activity as a specific target. Enzymes: Phospholipase C (EC 3.1.4.10).
