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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Predominant identification of RNA-binding proteins in Fas-induced apoptosis by proteome analysis

B Thiede1, C Dimmler, F Siejak

  • 1Max-Planck-Institut für Infektionsbiologie, Abteilung Molekulare Biologie, Schumannstr. 21/22, D-10117 Berlin, Germany.

The Journal of Biological Chemistry
|May 16, 2001
PubMed

Insights

Researchers identified 21 proteins modified during apoptosis in Jurkat T cells, including several RNA-binding proteins previously unknown to be involved in this programmed cell death process.

Area of Science:

  • Proteomics
  • Cell Biology
  • Molecular Biology

Background:

  • Apoptosis is a critical cellular process with complex regulatory mechanisms.
  • Identifying proteins involved in apoptosis is crucial for understanding cell death pathways.

Purpose of the Study:

  • To identify proteins that undergo modification during apoptosis in Jurkat T cells.
  • To characterize the role of these modified proteins in the apoptotic process.

Main Methods:

  • Proteome analysis of apoptotic and non-apoptotic Jurkat T cells using two-dimensional gel electrophoresis.
  • Subtractive analysis to identify differentially expressed protein spots.
  • Peptide mass fingerprinting via matrix-assisted laser desorption/ionization mass spectrometry for protein identification.

Main Results:

  • 45 protein spots showed differences between apoptotic and non-apoptotic cells.
  • 21 distinct proteins were identified, with 37 protein spots characterized.
  • Proteins including hnRNPs, splicing factors, and RNA-binding proteins were found to be involved in apoptosis for the first time.
  • 15 of the 21 identified proteins contained RNA-binding motifs (RNP or KH).

Conclusions:

  • This study reveals novel proteins, particularly RNA-binding proteins, implicated in the apoptosis of Jurkat T cells.
  • The findings contribute to a deeper understanding of the molecular mechanisms governing programmed cell death.

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