Related Experiment Videos
Incorporation of an aldehyde function in oligonucleotides
J M Tilquin1, M Dechamps, E Sonveaux
1Laboratoire de Chimie Thérapeutique et de Radiopharmacie, Université Catholique de Louvain, 73, Avenue Emmanuel Mounier, p. b. CMFA 7340, B-1200 Bruxelles, Belgium.
Bioconjugate Chemistry
|May 17, 2001
Abstract:
A nucleotide-like phosphoramidite building block that has the nucleic base replaced by the tert-butyldimethylsilyl-protected styrene glycol was synthesized. After the automatic synthesis of an oligonucleotide incorporating this synthon, the benzaldehyde function was generated by fluoride deprotection and oxidation by sodium periodate. In a similar manner, an oligonucleotide where a nucleic base was replaced by the (CH2)8CH=O chain was synthesized and conjugated with biotin derivatives.