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Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a
I Bastian1, L Rigouts, J C Palomino
1Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. ivan.bastien@imvs.sa.gov.au
Antimicrobial Agents and Chemotherapy
|May 17, 2001
Abstract:
Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 microg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 microg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 microg of kanamycin/ml. Both systems provided results within an average of 1 week.