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HIV Rev peptides conjugated with peptide nucleic acids and their efficient binding to RRE RNA
I Kumagai1, T Takahashi, K Hamasaki
1Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan.
Bioorganic & Medicinal Chemistry Letters
|May 17, 2001
Abstract:
HIV Rev peptides conjugated with peptide nucleic acids (PNAs) were designed and synthesized to develop a designing approach for a novel RNA-binding molecule. The binding affinities of PNA-peptides with the Rev responsive element (RRE) RNA were determined by the competition assay using a rhodamine-labeled Rev. The peptide conjugated with an antisense PNA (TGCGC) bound RRE RNA more efficiently than the molecule without the PNA or the peptide sequence.