Defective activity and isoform of the Na,K-ATPase in the dilated cardiomyopathic hamster

Related Experiment Video

Updated: Oct 7, 2026

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
12:48

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

F Paganelli1, R Mougenot, J M Maixentt

  • 1Department of Cardiology, H pital Nord, Marseille, France.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|May 17, 2001
PubMed

Insights

Dilated cardiomyopathy in hamsters shows reduced sodium-potassium adenosine triphosphatase (Na,K-ATPase) activity and specific isoform changes, mirroring findings in human heart failure.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Biochemistry

Background:

  • Sodium-potassium adenosine triphosphatase (Na,K-ATPase) is crucial for cardiac function.
  • Its impairment is observed in human heart failure with dilation.
  • Limited information exists regarding Na,K-ATPase in animal models of idiopathic dilated cardiomyopathy.

Purpose of the Study:

  • To investigate Na,K-ATPase isoform composition and activity in a hamster model of dilated cardiomyopathy.
  • To compare these findings with healthy controls.

Main Methods:

  • Studied cardiomyopathic MS 200 hamsters and healthy Syrian hamsters.
  • Utilized isoform-specific antibodies to analyze Na,K-ATPase alpha and beta1 subunits in ventricular myocardium.
  • Quantified Na,K-ATPase activity in homogenate and membrane fractions.

Main Results:

  • Dilated cardiomyopathy in hamsters showed significantly reduced membrane alpha1 and beta1 isoform abundances.
  • Na,K-ATPase activity was reduced by 35% compared to healthy controls (p<0.05).
  • No significant changes were observed in the Na,K-ATPase alpha2-subunit.

Conclusions:

  • Dilated cardiomyopathy in this hamster model specifically reduces membrane alpha1 and beta1 isoform abundance.
  • This reduction correlates with decreased Na,K-ATPase activity, similar to human dilated heart failure.
  • Provides a relevant animal model for studying Na,K-ATPase dysfunction in heart failure.

More Related Videos

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes12:49

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

Published on: September 24, 2013

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes07:32

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

Published on: May 25, 2022

Related Concept Videos

ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
ATP Synthase: Structure01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...