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Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
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Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

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RT-PCR detection of the Na,K-ATPase beta3-isoform in human heart

S Pierre1, F Paganelli, S Sennoune

  • 1Laboratoire de Biochimie Fondamentale, Moléculaire et Clinique, UFR de Pharmacie, Marseille, France

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|May 17, 2001
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...