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Endogenous cardiotonic steroids.

W Schoner1

  • 1Institut für Biochemie und Endokrinologie, Fachbereich Veterinärmedizin, Justus-Liebig-Universität Giessen, Germany. wilhelm.schoner@vetmed.uni-giessen.de

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|May 17, 2001
PubMed
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Ouabain, an endogenous digitalis, is found in the blood and linked to hypertension. Its antagonists offer potential new therapies for high blood pressure and heart failure.

Area of Science:

  • Endocrinology
  • Cardiovascular Physiology
  • Pharmacology

Background:

  • The search for endogenous digitalis compounds has identified cardiotonic steroids like ouabain.
  • These steroids are present in blood and released by adrenal cells in response to angiotensin II.
  • Elevated ouabain levels are observed in some individuals with low renin hypertension.

Purpose of the Study:

  • To investigate the role of ouabain as an endogenous hormone in sodium metabolism.
  • To explore the therapeutic potential of ouabain antagonists in cardiovascular diseases.

Main Methods:

  • Isolation of ouabain from blood, adrenal, and hypothalamus.
  • Culturing adrenal cortical cells to observe ouabain release.
  • Measuring blood ouabain levels in hypertensive patients.

Related Experiment Videos

  • Assessing the effects of PST 2238 (cardiac glycoside antagonist) on hypertension in rats.

    • Main Results:

    • Ouabain was isolated and identified as a circulating cardiotonic steroid.
    • Adrenal cells release ouabain upon stimulation with angiotensin II.
    • Increased ouabain levels correlate with low renin hypertension in a subset of patients.
    • Ouabain promotes myocyte growth in vitro, potentially via sodium pump inhibition and calcium increase.
    • PST 2238 treatment reduced hypertension in rats.

    Conclusions:

    • Ouabain functions as an adrenal hormone involved in sodium metabolism.
    • Ouabain's role in myocyte growth suggests a mechanism for cardiovascular changes.
    • Ouabain antagonists present a promising therapeutic avenue for hypertension and heart failure.