Evaluation and application of the AmpF/STR profiler plus PCR amplification kit in a Bavarian population sample

Allele frequencies for the nine tetrameric STR loci D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 were determined in a population sample of 155 unrelated Bavarians using the AmpF/STR Profiler Plus PCR amplification kit. No deviations from the Hardy-Weinberg equilibrium were observed. The influence of the PCR cycle number as well as the template DNA concentration on the performance of the kit was studied. DNA concentrations lower than 75 pg DNA per 25 microliters reaction volume resulted in allelic drop-out.