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Rapid PCR-based detection of Brugia malayi DNA from blood spots by DNA Detection Test Strips

S Klüber1, T Supali, S A Williams

  • 1Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany.

Transactions of the Royal Society of Tropical Medicine and Hygiene
|May 18, 2001
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...