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Gene amplification and expression of the steroid receptor coactivator SRC3 (AIB1) in sporadic breast and endometrial

M Glaeser1, T Floetotto, B Hanstein

  • 1Department of Gynecology and Obstetrics, Heinrich-Heine-University, Duesseldorf, Germany.

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|May 18, 2001
PubMed

Insights

Steroid receptor coactivator 3 (SRC3) overexpression is present in breast and endometrial cancers but not due to gene amplification. Further research is needed to understand its carcinogenic role.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Steroid receptor coactivator 3 (SRC3) acts as a coactivator for nuclear receptors, potentially enhancing their effects when overexpressed.
  • Gene amplification is a known cause of overexpression for oncogenes, but its role in SRC3-related cancers is unclear.

Purpose of the Study:

  • To investigate SRC3 gene amplification and expression levels in breast and endometrial carcinomas.
  • To determine if SRC3 gene amplification is the cause of SRC3 overexpression in these cancers.

Main Methods:

  • Quantitative differential PCR and RT-PCR were used to analyze gene amplification and mRNA expression.
  • Samples included 127 sporadic breast carcinomas, 30 endometrial carcinomas, and various cancer cell lines.

Main Results:

  • SRC3 gene amplification was not detected in breast or endometrial carcinomas but was found in MCF7 (10-fold) and HeLa (3-fold) cell lines.
  • SRC3 overexpression was observed in 13% of breast carcinomas and 17% of endometrial carcinomas.
  • Overexpression in tumors was not linked to gene amplification.

Conclusions:

  • SRC3 overexpression in breast and endometrial tumors is not caused by SRC3 gene amplification.
  • The carcinogenic potential of SRC3 overexpression requires further investigation.

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