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Updated: Aug 15, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Published on: April 10, 2020
Microsomal malonyl-CoA-sensitive carnitine acyltransferase
N M Broadway1, R J Pease, G Birdsey
1Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U.K.
Abstract:
Liver microsomes contain two carnitine acyltransferase activities. One of these has properties closely corresponding to those of 88 kDa mitochondrial carnitine palmitoyltransferase-1 (CPT-1). Antisera against CPT-1 cross-react with an 88 kDa microsomal protein, suggesting that CPT-1 may be targeted to both microsomal and mitochondrial membranes. However, no experiments using cDNAs corresponding to CPT-1 involving in vitro translation with microsomes or involving in vivo COS-1 cell transfection provided any evidence to support this hypothesis.
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