Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell adhesion molecule expression in the sheep thymus.

T Zhao1, C He, M Su

  • 1Laboratory of Immunophysiology, the Dana-Farber Cancer Institute, Harvard Surgical Research Laboratories, Harvard Medical School, 02115, Boston, MA, USA.

Developmental and Comparative Immunology
|May 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous determination of electrical conductivity and thickness of nonmagnetic metallic foils using two-sided multifrequency eddy current techniques.

The Review of scientific instruments·2023
Same author

Habituation and individual variation in the endocrine stress response in the Trinidadian guppy (Poecilia reticulata).

General and comparative endocrinology·2018
Same author

Nasal and sinus symptoms and chronic rhinosinusitis in a population-based sample.

Allergy·2016
Same author

Fundamental problems with the cooperative breeding hypothesis. A reply to Burkart & van Schaik.

Journal of zoology (London, England : 1987)·2016
Same author

Observation of B→D(*) π^(+)π^(-)ℓ^(-)ν Decays in e^(+)e^(-) Collisions at the Υ(4S) Resonance.

Physical review letters·2016
Same author

Elevated glucocorticoid concentrations during gestation predict reduced reproductive success in subordinate female banded mongooses.

Biology letters·2015

Researchers developed sheep monoclonal antibodies (mAbs) to study T cell development. Enhanced expression of beta1 integrin, ICAM-2, and LFA-3 was found on cortical thymocytes, aiding future research.

Area of Science:

  • Immunology
  • Developmental Biology
  • Veterinary Science

Background:

  • Cell adhesion molecules play a crucial role in T cell development within the thymus.
  • A large animal model is needed for in vivo studies of intrathymic T cell development and lymphocyte migration.
  • Previous studies on thymic development require further investigation using advanced tools.

Purpose of the Study:

  • To develop a panel of monoclonal antibodies (mAbs) against sheep cell adhesion molecules.
  • To characterize the expression of cell adhesion molecules on sheep thymocytes using flow cytometry.
  • To identify specific cell adhesion molecules involved in intrathymic T cell development in sheep.

Main Methods:

  • Development of monoclonal antibodies (mAbs) to various sheep cell adhesion molecules.

Related Experiment Videos

  • Immunohistochemistry to determine mAb reactivity.
  • Flow cytometry, including dual parameter analysis with anti-sheep CD1 antigen mAbs, to quantify cell adhesion molecule expression on thymocyte subsets.

    • Main Results:

    • Significantly enhanced expression of beta1 integrin (CD29), ICAM-2, and LFA-3 on cortical thymocytes was observed.
    • Beta1 integrin (CD29) showed higher expression on CD1(+) thymocytes in post-natal lambs compared to adult sheep.
    • Phenotypic characterization revealed differential expression patterns of cell adhesion molecules on CD1(+) and CD1(-) thymocyte populations.

    Conclusions:

    • The developed mAbs and characterization of cell adhesion molecule expression provide a valuable resource for studying sheep T cell development.
    • Enhanced expression of specific cell adhesion molecules on cortical thymocytes suggests their involvement in intrathymic T cell development.
    • These findings facilitate future in vivo investigations of sheep intrathymic development and T lymphocyte immigration.