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Related Experiment Videos

Modelling plasmid instability in batch and continuous fermentors.

H R. Baheri1, G A. Hill, W J. Roesler

  • 1Department of Chemical Engineering and Petroleum Engineering, 2500 University Drive NW, AB, T2N 1N4, Calgary, Canada

Biochemical Engineering Journal
|May 18, 2001
PubMed
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This study models plasmid loss in growing cells within bioreactors. The new model predicts varying rates of plasmid loss in batch and continuous cultures, aligning with experimental data.

Area of Science:

  • Biotechnology
  • Microbial genetics
  • Chemical engineering

Background:

  • Plasmid loss during cell growth can impact biotechnological processes.
  • Existing models for plasmid loss dynamics have limitations in complexity or ease of use.

Purpose of the Study:

  • To develop and validate a new, user-friendly model for predicting plasmid loss in plasmid-bearing cells.
  • To incorporate the probability of complete plasmid loss into dynamic cell concentration predictions in bioreactors.

Main Methods:

  • Mathematical modeling based on an extension of the Imanaka and Aiba model.
  • Application of the model to predict plasmid-bearing cell concentrations in batch and continuous stirred tank bioreactors.

Main Results:

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  • The model predicts both accelerating and decelerating rates of plasmid loss.
  • Model predictions show good agreement with experimental data from this study and previous research.

Conclusions:

  • The developed model provides a practical approach to understanding and predicting plasmid loss dynamics.
  • This model can aid in optimizing bioreactor operations for processes reliant on plasmid-bearing cells.