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Anesthetic protocol: propofol use in Rhesus macaques (Macaca mulatta) during magnetic resonance imaging with
K A Fowler1, M J Huerkamp, J K Pullium
1Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA. kfowler@dar.emory.edu
Brain Research. Brain Research Protocols
|May 18, 2001
Summary
Intravenous propofol anesthesia in Rhesus monkeys enables precise MRI-guided stereotactic intracranial surgery. This method overcomes challenges of magnetic resonance imaging (MRI) and stereotactic procedures, ensuring minimal complications.
Area of Science:
- Neuroscience
- Veterinary Medicine
- Medical Imaging
Background:
- Accurate targeting for stereotactic intracranial surgery in research animals often requires Magnetic Resonance Image (MRI) guidance.
- Challenges include the MR imaging equipment's magnetic field, stereotactic head frame logistics, and subject movement limitations.
Purpose of the Study:
- To evaluate the utility of intravenous propofol for anesthetizing Rhesus monkeys for high-resolution 3D MRI.
- To assess the feasibility of transitioning to inhalation anesthesia for subsequent stereotactic surgery with the head frame in place.
Main Methods:
- Adult Rhesus monkeys were anesthetized intravenously with propofol.
- High-resolution 3D MRI scans were acquired immediately after induction.
- Anesthesia was converted to inhalation for stereotactic intracranial surgery with the head frame 'in situ.'
Main Results:
- Intravenous propofol facilitated high-resolution 3D MRI acquisition in Rhesus monkeys.
- The transition to inhalation anesthesia for stereotactic surgery was successful with the head frame maintained.
- The procedure resulted in minimal morbidity and high precision for stereotactic targeting.
Conclusions:
- Intravenous propofol is a useful anesthetic agent for MRI-guided stereotactic procedures in Rhesus monkeys.
- This anesthetic approach effectively addresses the unique challenges of MRI and stereotactic surgery in research settings.
- The method allows for precise targeting with minimal adverse effects in animal models.