Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gonadotropins decrease estrogen receptor-beta messenger ribonucleic acid stability in rat granulosa cells.

C Guo1, L Savage, K D Sarge

  • 1Department of Physiology, University of Kentucky, Lexington 40536-0084, USA.

Endocrinology
|May 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum: "Highly versatile, two-color setup for high-order harmonic generation using spatial light modulators" [Rev. Sci. Instrum. 95, 073002 (2024)].

The Review of scientific instruments·2026
Same author

Deep learning-based automated assessment of pulmonary artery indices and surgical approach triage for tetralogy of Fallot from multicenter cardiac computed tomography (CT).

Clinical radiology·2026
Same author

The effects of nitrogen and phosphorus fertilization on nutrient resorption in female and male Populus cathayana.

Plant biology (Stuttgart, Germany)·2026
Same author

Rubisco large and small subunits provide insights into allotetraploidy events from the genus Oryza.

Plant biology (Stuttgart, Germany)·2026
Same author

Energy Bunching from Subcycle Ionization Injection in Laser Wakefield Acceleration.

Physical review letters·2026
Same author

Dimensionality of the reinforced superconductivity in UTe<sub>2</sub>.

Nature communications·2025
Same journal

Zingerone ameliorates ovarian impairment by regulating steroidogenesis and apoptosis in letrozole-treated mouse.

Endocrinology·2026
Same journal

Loss of the calorie restriction response protein DEPP1 worsens diet-induced obesity.

Endocrinology·2026
Same journal

Long Non-Coding RNAs Mediate Endocrine Signaling and Resistance in Prostate Cancer.

Endocrinology·2026
Same journal

Repeated Chemogenetic Activation of C1 Catecholamine Neurons Reduces Subsequent Glucoprivic Responses and Mimics HAAF.

Endocrinology·2026
Same journal

Postnatal exposure to maternal hypothyroidism leads to developmental delay and metabolic dysregulations in male mice.

Endocrinology·2026
Same journal

Discovery proteomics identification of factors contributing to gonadotropin β expression.

Endocrinology·2026
See all related articles

The luteinizing hormone (LH) surge reduces estrogen receptor-beta (ERbeta) mRNA in granulosa cells by destabilizing existing mRNA, not by altering gene transcription. This process is translation-dependent and sensitive to protein synthesis inhibition.

Area of Science:

  • Reproductive Endocrinology
  • Molecular Biology
  • Cellular Signaling

Background:

  • The preovulatory LH surge plays a critical role in ovulation.
  • Estrogen receptor-beta (ERbeta) mRNA levels in granulosa cells are known to be downregulated by the LH surge.
  • The precise molecular mechanisms underlying this downregulation remain to be fully elucidated.

Purpose of the Study:

  • To investigate whether the LH-induced decrease in ERbeta mRNA in rat granulosa cells occurs at the transcriptional or posttranscriptional level.
  • To determine if mRNA degradation or altered transcription is the primary mechanism for LH-mediated ERbeta mRNA reduction.
  • To explore the role of translation in the LH-induced destabilization of ERbeta mRNA.

Main Methods:

  • Nuclear run-off assays to assess ERbeta gene transcription rates.

Related Experiment Videos

  • Culture of granulosa cells with RNA synthesis inhibitor (DRB) to evaluate mRNA stability.
  • Treatment with hCG, forskolin (FSK), and TPA to mimic LH actions.
  • Semiquantitative RT-PCR and linear regression analysis to determine ERbeta mRNA half-life.
  • Experiments using cycloheximide (protein synthesis inhibitor) to assess translation dependence.

    • Main Results:

    • LH (hCG) had a marginal effect on ERbeta gene transcription.
    • LH (hCG) significantly reduced ERbeta mRNA levels, primarily through mRNA destabilization.
    • The half-life of ERbeta mRNA was significantly decreased by hCG, FSK, and TPA.
    • The inhibitory effects of LH on ERbeta mRNA levels were abolished by cycloheximide, indicating a requirement for protein synthesis.

    Conclusions:

    • LH decreases ERbeta mRNA expression in granulosa cells mainly by enhancing mRNA degradation (posttranscriptional regulation).
    • The LH-induced destabilization of ERbeta mRNA is a translation-dependent process.
    • These findings provide crucial insights into the molecular mechanisms regulating ERbeta expression during the preovulatory period.